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  • 杨明磊,李逸明,陈辉莹,李凯利,王琰,马雅军,杨振洲,詹道成.我国西沙群岛吸血昆虫的种群动态和鼠形动物病原感染检测[J].第二军医大学学报,2016,37(3):355-359    [点击复制]
  • YANG Ming-lei,LI Yi-ming,CHEN Hui-ying,LI Kai-li,WANG Yan,MA Ya-jun,YANG Zhen-zhou,ZHAN Dao-cheng.Population dynamics of the blood-sucking insects and detection of pathogens in the rodents from Xisha Islands of China[J].Acad J Sec Mil Med Univ,2016,37(3):355-359   [点击复制]
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我国西沙群岛吸血昆虫的种群动态和鼠形动物病原感染检测
杨明磊1,李逸明1,陈辉莹2,李凯利2,王琰2,马雅军2*,杨振洲3*,詹道成4
0
(1. 第二军医大学学员旅学员一队, 上海 200433;
2. 第二军医大学热带医学与公共卫生学系热带传染病学教研室, 上海 200433;
3. 军事医学科学院疾病预防控制所, 北京 100071;
4. 解放军91922部队卫生防疫所, 三亚 572000
*通信作者)
摘要:
目的 研究我国西沙群岛吸血昆虫的种群动态以及鼠形动物病原感染情况, 为当地传染病防控提供资料。 方法 2014年1月至7月在西沙群岛的永兴岛和石岛以灯诱法诱捕昆虫, 每个月2次。结合形态和分子特征对昆虫进行种类鉴定并统计种群数量。用胶体金试剂条和PCR检测鼠形动物的常见病原感染情况, 依据形态学初步鉴定鼠形动物体外寄生虫。 结果 西沙群岛的吸血昆虫中, 库蚊、阿蚊和库蠓为优势种群, 蠓在4月密度最高(55.55%, 6 984/12 573), 并在西沙群岛采集到贝氏司蛉(n=11)。鼠形动物血清中A型金黄色葡萄球菌肠毒素和A型肉毒毒素的阳性检出率分别为3.45%(1/29)和14.00%(7/50), 鼠疫和立克次体病抗体均为阴性。巢氏PCR扩增70只鼠形动物的立克次体Sta58基因片段, 阳性率为11.43%(8/70)。获得鼠形动物体外寄生虫共248个个体, 其中革螨占93.55%(232/248)。 结论 吸血昆虫在西沙群岛未见明显的种群动态变化, 本研究首次记述了西沙群岛的白蛉;鼠形动物中恙虫病立克次体和2种细菌的毒素携带率较高。
关键词:  西沙群岛  吸血昆虫  白蛉  恙虫病立克次体
DOI:10.16781/j.0258-879x.2016.03.0355
投稿时间:2015-11-23修订日期:2016-01-27
基金项目:国家自然科学基金面上项目(81371848, 81172730), 第二军医大学大学生创新能力培养基金(MS2014001, MS2015009).
Population dynamics of the blood-sucking insects and detection of pathogens in the rodents from Xisha Islands of China
YANG Ming-lei1,LI Yi-ming1,CHEN Hui-ying2,LI Kai-li2,WANG Yan2,MA Ya-jun2*,YANG Zhen-zhou3*,ZHAN Dao-cheng4
(1. No. 1 Team of Undergraduate Brigade, Second Military Medical University, Shanghai 200433, China;
2. Department of Tropical Infectious Diseases, Faculty of Tropical Medicine and Public Health, Second Military Medical University, Shanghai 200433, China;
3. Institute of Disease Prevention and Control, Academy of Military Medical Sciences, Beijing 100071, China;
4. Department of Health and Epidemic Prevention, No. 91922 Troop of PLA, Sanya 572000, Hainan, China
*Corresponding author)
Abstract:
Objective To investigate the population dynamics of blood-sucking insects and detect the pathogen of rodents in Xisha Islands of China, so as to provide information for prevention and control of infectious diseases in the area. Methods From January to July in 2014, we collected blood-sucking insects in Yongxing and Shi Islands twice a month using light traps. The specimens were identified by morphologic characters and molecular markers. Meanwhile, the population size was calculated. The pathogenic infections of rodents were detected by immuno-colloidal gold chromatographic test strips and PCR assay. And the ectoparasites in the rodents were initially identified by morphologic characters. Results The dominant species of blood-sucking insects in Xisha Islands were species of Genus Culex, Armigeres and Culicoides, and in April the population density of biting midge was the highest (55.55%, 6 984/12 573). Phlebotomine sandfly specimen was also collected and identified as Sergentomyia baily (n=11) by mtDNA-COⅠ sequences. The positive rates of Staphylococcus aureus enterotoxin type A and Botulinum toxin type A were 3.45% (1/29) and 14.00% (7/50) in the rodent serum samples, while all the samples were negative for Tsutsugamushi disease and Plague antibodies. Sta58 gene of Rickettsia tsutsugamushi was amplified by nested PCR in 70 rodent's spleen tissues, with the positive rate being 11.43% (8/70). A total of 248 ectoparasites were collected, and 93.55% (232/248) of them was gamasid mites. Conclusion The population dynamics of blood-sucking insects has no obvious change. Phlebotomine sandflies has been first recorded in Xisha Islands. The infection rates by Rickettsia tsutsugamushi and two bacterial toxins are high.
Key words:  Xisha Islands  blood-sucking insects  Phlebotomine sandfly  Rickettsia tsutsugamushi