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  • 叶美华,方庆全*,张翠霞.一种石蜡切片降温组件在病理切片中的应用[J].第二军医大学学报,2019,40(11):1280-1282    [点击复制]
  • YE Mei-hua,FANG Qing-quan*,ZHANG Cui-xia.Application of a cooling module of paraffin section in pathological section[J].Acad J Sec Mil Med Univ,2019,40(11):1280-1282   [点击复制]
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一种石蜡切片降温组件在病理切片中的应用
叶美华,方庆全*,张翠霞
0
(厦门大学附属第一医院病理科, 厦门 361003
*通信作者)
摘要:
目的 探讨一种石蜡切片的降温组件在病理切片中的应用价值。方法 随机选取包埋好的100个蜡块,病理科技术人员先将100个蜡块进行粗修后备用。该100个蜡块先后分别用自己制作的冰块、冷冻台及降温组件进行冷却后再切片,将切片依次编为甲组、乙组及丙组,按同一程序进行染色。以十分制评定厚薄均匀、无皱褶、无裂隙3项指标的得分,3组切片分别进行比较。并分析3组切片在切片时效性的差异(进行10次测试)以及资金投入方面的差异。结果 ①甲组与乙组比较,厚薄均匀、无皱褶、无裂隙指标得分的差异有统计学意义(P均<0.01);甲组与丙组比较,无皱褶指标得分的差异有统计学意义(P<0.01),厚薄均匀、无裂隙指标得分的差异均无统计学意义(P均>0.05);乙组与丙组比较,厚薄均匀、无皱褶、无裂隙指标得分的差异均有统计学意义(P均<0.01)。②甲组切片耗时65.30±1.44分钟,乙组切片耗时79.80±1.95分钟,丙组切片耗时62.50±2.17分钟。甲组与乙组比较,差异有统计学意义(t=5.977,P<0.01);乙组与丙组比较,差异有统计学意义(t=5.930,P<0.01);甲组与丙组比较,差异无统计学意义(t=1.007,P>0.05)。③乙组6年资金投入约为7000元;6年期间甲组自己制作的冰块的成本是一台冰箱、一个不锈钢盘的成本以及盛放的水的成本大约3500元;丙组石蜡切片降温组件的成本与甲组的成本相当,比乙组的资金投入少。结论 在病理常规石蜡切片过程中,应用石蜡切片降温组件对蜡块进行冷却后进行切片,有着切片质量好、效率高、操作简便等优点,值得推荐。
关键词:  石蜡切片  冰块  冷冻台  降温组件
DOI:10.16781/j.0258-879x.2019.11.1280
投稿时间:2019-06-26修订日期:2019-10-09
基金项目:
Application of a cooling module of paraffin section in pathological section
YE Mei-hua,FANG Qing-quan*,ZHANG Cui-xia
(Department of Pathology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, Fujian, China
*Corresponding author)
Abstract:
Objective Discussion on the application value of a cooling module of paraffin section in pathological section.Methods Random selection of 100 paraffin blocks, the pathology technicians first roughed 100 wax pieces and then set them aside. The 100 pieces of wax were respectively sliced up and cooled with ice block made by themselves, frozen table and cooling module. The sections were successively divided into group A, group B and group C, and dyed according to the same program. The scores of the three indexes, such as thickness uniformity, no wrinkles and no crack, were evaluated by a 10-point scale, respectively.The three groups were compared with each other. The differences between the 3 groups of slices in timeliness(carry on 10 tests)and capital input were also analyzed. Results ①There were statistically significant differences in the scores of thickness uniformity, no wrinkles and no crack index(P<0.01) between group A and group B. There was significant difference in the score of no wrinkle index(P<0.01) while there were no significant difference in the scores of thickness uniformity or no crack index (P>0.05)between group A and C.There were statistically significant differences in the scores of thickness uniformity, no wrinkles and no crack index(P<0.01) between group B and group C. ② Group A slices took 65.30±1.44 minutes, group B slices took 79.80±1.95 minutes, and group C slices took 62.50±2.17 minutes. The difference between group A and B was statistically significant(t=5.977,P<0.01). The difference between group B and C was statistically significant(t=5.930,P<0.01). There was no statistical difference between group A and C (t=1.007,P>0.05).③The cost of group B in six years was about 7000 yuan.The cost of group A that the ice made by themselves in six years was about 3500 yuan for a refrigerator, a stainless steel plate, and water. The cost of paraffin section cooling module of group C was the same as that of group A, less than that of group B. Conclusion In the process of pathological routine paraffin section, the cooling module of paraffin section was used to slice the wax block after cooling, which had the advantages of good slice quality, high efficiency, simple operation and so on, which was worthy of recommendation.
Key words:  paraffin section  ice block  frozen table  cooling module