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  • 闫磊,章建全,陈红琼,吴震中,程杰.甲状腺结节微波消融治疗后6个月消融区细胞活性的酶组织化学检测[J].第二军医大学学报,2019,40(1):20-24    [点击复制]
  • YAN Lei,ZHANG Jian-quan,CHEN Hong-qiong,WU Zhen-zhong,CHENG Jie.Enzyme histochemical detection of cell viability in ablation area of thyroid nodules at 6 months after microwave ablation[J].Acad J Sec Mil Med Univ,2019,40(1):20-24   [点击复制]
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甲状腺结节微波消融治疗后6个月消融区细胞活性的酶组织化学检测
闫磊1,2△,章建全1,3△*,陈红琼3,吴震中1,程杰3
0
(1. 海军军医大学(第二军医大学)长征医院超声诊疗科, 上海 200003;
2. 解放军100医院超声科, 苏州 215007;
3. 上海国际医学中心, 上海 201318
共同第一作者
*通信作者)
摘要:
目的 探讨甲状腺结节微波消融治疗术后6个月消融区组织内细胞活性情况。方法 选择2017年12月至2018年9月甲状腺结节微波消融治疗术后6个月进行消融区粗针穿刺活组织检查病理评估的患者20例24个消融区。使用切割式活检针分别对消融区中央域及边缘域进行粗针穿刺活组织检查,取得标本条置入液氮制成冰冻切片。采用酶组织化学染色法检测细胞内琥珀酸脱氢酶(SDH)和还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶(NADPH-d)的活性,并与常规病理切片H-E染色观察到的细胞形态及组织结构对比。结果 微波消融术后6个月24个消融区的中央域及边缘域均成功取材。消融区中央域SDH及NADPH-d酶组织化学染色情况一致性好,阴性率均为95.83%(23/24)。消融区边缘域SDH及NADPH-d酶组织化学染色情况一致性好,阴性率均为91.67%(22/24)。23个中央域及22个边缘域的H-E染色切片均显示为红染无结构的大片坏死组织;1个中央域、2个边缘域H-E染色切片显示部分为坏死结构、部分为纤维组织增生,纤维组织增生处与酶组织化学染色阳性区域位置一致。结论 微波消融术后6个月甲状腺结节消融区组织符合凝固性坏死改变,仍处于失活状态,酶组织化学染色结合H-E染色能够对陈旧消融区做出较为客观的评价。
关键词:  甲状腺结节  热消融术  酶组织化学染色  琥珀酸脱氢酶  还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶
DOI:10.16781/j.0258-879x.2019.01.0020
投稿时间:2018-06-04修订日期:2018-11-29
基金项目:国家自然科学基金(81171436).
Enzyme histochemical detection of cell viability in ablation area of thyroid nodules at 6 months after microwave ablation
YAN Lei1,2△,ZHANG Jian-quan1,3△*,CHEN Hong-qiong3,WU Zhen-zhong1,CHENG Jie3
(1. Department of Ultrasound, Changzheng Hospital, Naval Medical University(Second Military Medical University), Shanghai 200003, China;
2. Department of Ultrasound, No. 100 Hospital of PLA, Suzhou 215007, Jiangsu, China;
3. Shanghai International Medical Center, Shanghai 201318, China
Co-first authors.
* Corresponding author)
Abstract:
Objective To explore the cell viability in the ablation area of thyroid nodules at 6 months after microwave ablation by enzyme histochemical staining.Methods Twenty-four ablation areas of thyroid nodules were selected from 20 patients who underwent histopathological assessment of the ablation area by core needle biopsy at 6 months after microwave ablation between Dec. 2017 and Sep. 2018. Core needle biopsy was performed at the central and marginal regions of the ablation area with a cutting biopsy needle. The specimens were obtained and placed in liquid nitrogen to make frozen sections. Enzyme histochemical staining was used to detect the activities of succinate dehydrogenase (SDH) and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d), and the difference of cell morphology and histological structure was compared with H-E staining results.Results The specimens of the central and marginal regions of 24 ablation areas were successfully obtained. The histochemical staining of SDH and NADPH-d in the central region of ablation area had good consistency, and the negative rates were both 95.83% (23/24). The histochemical staining of SDH and NADPH-d in the marginal region of ablation area also had good consistency, and the negative rates were both 91.67% (22/24). H-E staining of 23 central regions and 22 marginal regions showed pink amorphous mass of necrosis. H-E staining of 1 central region and 2 marginal regions showed partly necrotic and fibrous tissue hyperplasia. The location of fibrous tissue hyperplasia was consistent with the location of the positive region of enzyme histochemical staining.Conclusion At 6 months after microwave ablation, the tissue in the ablation area of thyroid nodules is consistent with coagulative necrosis, and is still inactivated. SDH or NADPH-d enzyme histochemical staining combined with H-E staining can objectively evaluate the old ablation area.
Key words:  thyroid nodule  thermal ablation  enzyme histochemical staining  succinate dehydrogenase  nicotinamide adenine dinucleotide phosphate diaphorase