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  • 衣洪杰,郑成刚*.高压氧对妊娠大鼠一氧化碳中毒的疗效及对子代学习记忆能力的影响[J].第二军医大学学报,2019,40(4):408-411    [点击复制]
  • YI Hong-jie,ZHENG Cheng-gang*.Therapeutic effect of hyperbaric oxygen on carbon monoxide poisoning in pregnant rats and its effect on learning and memory ability of their offspring[J].Acad J Sec Mil Med Univ,2019,40(4):408-411   [点击复制]
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高压氧对妊娠大鼠一氧化碳中毒的疗效及对子代学习记忆能力的影响
衣洪杰,郑成刚*
0
(海军军医大学(第二军医大学)长海医院高压氧治疗科, 上海 200433
*通信作者)
摘要:
目的 观察高压氧(HBO)对妊娠大鼠一氧化碳(CO)中毒的疗效及对子代大鼠学习记忆能力的影响。方法 将120只SD孕鼠(E3)随机分为4组:对照组、CO中毒组、HBO治疗组和常压氧(NBO)治疗组,对照组不作处理,后3组进行急性CO中毒造模,HBO治疗组造模成功后以O2浓度≥99%的HBO(绝对压250 kPa)治疗至大鼠生产,NBO治疗组造模成功后以O2浓度≥99%的NBO治疗至大鼠生产。记录孕鼠死亡率、孕鼠产仔数量、仔鼠体质量、仔鼠死亡率,通过Morris水迷宫实验评估子代大鼠的学习记忆能力。结果 与CO中毒组比较,HBO治疗组孕鼠和仔鼠的死亡率均降低[0 vs 14.8%(4/27),P<0.01;1.0%(3/292)vs 8.6%(18/209),P<0.05],孕鼠产仔数量和仔鼠体质量均增加[(11.2±1.6)只vs(9.1±2.1)只,P<0.05;(6.55±1.13)g vs(5.27±1.02)g,P<0.05],子代大鼠学习记忆能力增强(水迷宫躲避潜伏期缩短,P<0.01)。NBO治疗组上述指标与CO中毒组比较也有明显改善,但较HBO治疗组效果差(P<0.05)。结论 HBO和NBO治疗对妊娠大鼠CO中毒均有一定疗效,并能减轻CO中毒对子代大鼠学习记忆能力的影响,且HBO治疗效果好于NBO。
关键词:  一氧化碳中毒  高压氧  妊娠  记忆
DOI:10.16781/j.0258-879x.2019.04.0408
投稿时间:2018-10-19修订日期:2019-03-22
基金项目:
Therapeutic effect of hyperbaric oxygen on carbon monoxide poisoning in pregnant rats and its effect on learning and memory ability of their offspring
YI Hong-jie,ZHENG Cheng-gang*
(Department of Hyperbaric Oxygen, Changhai Hospital, Naval Medical University(Second Military Medical University), Shanghai 200433, China
*Corresponding author)
Abstract:
Objective To explore the curative effect of hyperbaric oxygen (HBO) on carbon monoxide (CO) poisoning of pregnant rats and its effect on learning and memory ability of the offspring rats. Methods A total of 120 pregnant SD rats (E3) were randomly divided into four groups:control, CO poisoning, CO poisoning+HBO and CO poisoning+normobaric oxygen (NBO) groups. The rats in the control group were not treated, while those in the other 3 groups were treated with acute CO poisoning. After successful modelling, the rats in the HBO group were treated with HBO (O2 ≥ 99%, absolute pressure 250 kPa) till delivery, and the rats in the NBO group were treated with NBO (O2 ≥ 99%, normal pressure) till delivery. The mortality rate of pregnant rats, the number of pups, the body mass of the pups and the mortality rate of pups were recorded, and the memory and learning ability of the offspring rats was assessed with Morris water maze. Results Compared with the CO poisoning group, HBO treatment could significantly reduce the mortality rate of pregnant rats and pups (0 vs 14.8%[4/27], 1.0%[3/292] vs 8.6%[18/209]; P<0.01, P<0.05), increase the number of pups and the body mass of pups (11.2±1.6 vs 9.1±2.1,[6.55±1.13] g vs[5.27±1.02] g; both P<0.05), and enhance the learning and memory ability of pups (escape avoidance latency was significantly shortened in Morris water maze, P<0.01). The above indicators were also improved after NBO treatment, but the effect was significantly lower than HBO treatment (P<0.05). Conclusion Both HBO and NBO can treat CO poisoning of pregnant rats, and can reduce the effects of CO poisoning on learning and memory ability of offspring rats, but the therapeutic effect of HBO treatment is better than NBO.
Key words:  carbon monoxide poisoning  hyperbaric oxygenation  pregnancy  memory