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  • 周洋,刘晓军,孙昊,吴玉仙*,金志军*.宫颈癌中活化的蛋白激酶C受体1、低氧诱导因子1α与血管内皮生长因子的表达及病理学意义[J].第二军医大学学报,2019,40(10):1078-1082    [点击复制]
  • ZHOU Yang,LIU Xiao-jun,SUN Hao,WU Yu-xian*,JIN Zhi-jun*.Expression of receptor for activated C kinase 1, hypoxia-inducible factor 1α, and vascular endothelial growth factor in cervical cancer and their pathological significance[J].Acad J Sec Mil Med Univ,2019,40(10):1078-1082   [点击复制]
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宫颈癌中活化的蛋白激酶C受体1、低氧诱导因子1α与血管内皮生长因子的表达及病理学意义
周洋,刘晓军,孙昊,吴玉仙*,金志军*
0
(海军军医大学(第二军医大学)长征医院妇产科, 上海 200003
*通信作者)
摘要:
目的 检测活化的蛋白激酶C受体1(RACK1)、低氧诱导因子1α(HIF-1α)、血管内皮生长因子(VEGF)在宫颈癌组织中的表达并探讨其病理学意义。方法 选取2014年6月至2018年6月于本院行手术切除并经病理确诊的85例宫颈癌组织样本及其对应的癌旁组织,通过免疫组织化学染色ABC法分别检测RACK1、HIF-1α、VEGF蛋白在宫颈癌组织及其癌旁组织中的表达。结合患者的临床病理资料,分析宫颈癌组织中RACK1、HIF-1α和VEGF蛋白表达与患者年龄及肿瘤直径、浸润深度、病理分级、淋巴结转移之间的关系,并分析三者表达之间的相关性。结果 RACK1、HIF-1α、VEGF蛋白在宫颈癌组织中的表达均高于癌旁组织(P均<0.05),阳性表达率分别为81.2%(69/85)、63.5%(54/85)、89.4%(76/85)。RACK1表达与肿瘤浸润深度、病理分级和淋巴结是否转移有关(P均<0.05),HIF-1α和VEGF表达均与肿瘤直径、浸润深度、病理分级及淋巴结是否转移有关(P均<0.05)。RACK1、HIF-1α、VEGF蛋白的表达两两之间呈正相关(RACK1 vs HIF-1α:r=0.523,P=0.043 9;RACK1 vs VEGF:r=0.428,P=0.033 7;HIF-1α vs VEGF:r=0.689,P=0.024 5)。结论 RACK1、HIF-1、VEGF蛋白在宫颈癌组织中高表达且三者之间的表达呈正相关,提示其可能在肿瘤的发生、发展过程中起协同作用,可作为判断肿瘤侵袭、转移及预后的重要指标。
关键词:  宫颈肿瘤  活化的蛋白激酶C受体1  低氧诱导因子1α  血管内皮生长因子
DOI:10.16781/j.0258-879x.2019.10.1078
投稿时间:2019-02-01修订日期:2019-07-14
基金项目:
Expression of receptor for activated C kinase 1, hypoxia-inducible factor 1α, and vascular endothelial growth factor in cervical cancer and their pathological significance
ZHOU Yang,LIU Xiao-jun,SUN Hao,WU Yu-xian*,JIN Zhi-jun*
(Department of Gynaecology and Obstetrics, Changzheng Hospital, Naval Medical University(Second Military Medical University), Shanghai 200003, China
*Corresponding authors)
Abstract:
Objective To detect the expression of receptor for activated C kinase 1 (RACK1), hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in cervical cancer, and to explore their pathological significance. Methods Eighty-five cervical cancer samples and their corresponding paracancerous tissues, which were surgically removed and confirmed by pathology in our hospital from Jun. 2014 to Jun. 2018, were included in this study. The protein expression levels of RACK1, HIF-1α and VEGF were detected by immunohistochemical staining. The relationship between the protein expression of RACK1, HIF-1α and VEGF in cervical cancer tissues and clinicopathological features, including age, tumor diameter, invasion depth, clinical stage and lymph node metastasis, were analyzed, and the correlation between the expression of RACK1, HIF-1α and VEGF was analyzed. Results Compared with paracancerous tissues, the protein expression levels of RACK1, HIF-1α and VEGF in cervical cancer tissues were significantly higher (all P<0.05), with the positive rates being 81.2% (69/85), 63.5% (54/85), and 89.4% (76/85), respectively. The expression of RACK1 was associated with clinical stage, invasion depth and lymph node metastasis (all P<0.05). The expression levels of HIF-1α and VEGF were associated with the tumor diameter, invasion depth, clinical stage and lymph node metastasis (all P<0.05). The expression levels of RACK1, HIF-1, VEGF proteins were positively correlated with each other (RACK1 vs HIF-1α, r=0.523, P=0.043 9; RACK1 vs VEGF, r=0.428, P=0.033 7; HIF-1α vs VEGF, r=0.689, P=0.024 5). Conclusion RACK1, HIF-1α and VEGF are highly expressed in cervical cancer and their expression levels are positively correlated, suggesting that they may play a synergistic role in the development and progression of cervical cancer, and can be an important marker for predicting the invasion, metastasis and prognosis.
Key words:  uterine cervical neoplasms  receptor for activated C kinase 1  hypoxia-inducible factor 1α  vascular endothelial growth factor