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  • 王湘玥,黄英姿,耿邦尉,闵伟杰,王毛毛,李亚楠.长链非编码RNA MIR210HG促进胶质母细胞瘤生长及侵袭[J].第二军医大学学报,2020,41(6):661-667    [点击复制]
  • WANG Xiang-yue,HUANG ying-zi,GENG Bang-wei,MIN Wei-jie,WANG Mao-mao,LI Ya-nan.Long non-coding RNA MIR210HG promotes growth and invasion of glioblastoma[J].Acad J Sec Mil Med Univ,2020,41(6):661-667   [点击复制]
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长链非编码RNA MIR210HG促进胶质母细胞瘤生长及侵袭
王湘玥1,黄英姿1,耿邦尉2,闵伟杰3,王毛毛3,李亚楠3*
0
(1. 海军军医大学(第二军医大学)基础医学院学员三队, 上海 200433;
2. 海军军医大学(第二军医大学)基础医学院学员十二队, 上海 200433;
3. 海军军医大学(第二军医大学)长海医院神经外科, 上海 200433
*通信作者)
摘要:
目的 筛选胶质母细胞瘤中差异表达的长链非编码RNA(lncRNA),探讨其在胶质母细胞瘤生长及侵袭中的作用。方法 选取8例配对的胶质母细胞瘤瘤体组织与瘤周正常脑组织进行高通量杂交实验,筛选表达差异最显著的lncRNA,建立该lncRNA过表达和干扰表达的人胶质母细胞瘤细胞模型。利用CCK-8检测细胞增殖能力,流式细胞术检测细胞凋亡情况,Transwell实验检测细胞侵袭和迁移能力。结果 筛选出在胶质母细胞瘤中高表达的lncRNA MIR210HG,并针对lncRNA MIR210HG成功建立了过表达和干扰表达胶质母细胞瘤细胞株。体外实验发现,过表达lncRNA MIR210HG后胶质母细胞瘤细胞增殖能力增强、凋亡细胞比例下降、侵袭和迁移能力增强,干扰lncRNA MIR210HG表达后胶质母细胞瘤细胞增殖能力下降、凋亡细胞比例升高、侵袭和迁移能力下降,与对照组相比差异均有统计学意义(P均<0.05)。结论 LncRNA MIR210HG能促进胶质母细胞瘤细胞增殖、抑制凋亡,并增强细胞侵袭和迁移,有望成为胶质母细胞瘤治疗的新靶点。
关键词:  胶质母细胞瘤|长链非编码RNA|细胞增殖|细胞凋亡|细胞侵袭|细胞迁移
DOI:10.16781/j.0258-879x.2020.06.0661
投稿时间:2020-03-11修订日期:2020-06-06
基金项目:上海市自然科学基金(17ZR1438300).
Long non-coding RNA MIR210HG promotes growth and invasion of glioblastoma
WANG Xiang-yue1,HUANG ying-zi1,GENG Bang-wei2,MIN Wei-jie3,WANG Mao-mao3,LI Ya-nan3*
(1. The Third Student Team, College of Basic Medical Sciences, Naval Medical University (Second Military Medical University), Shanghai 200433, China;
2. The Twelfth Student Team, College of Basic Medical Sciences, Naval Medical University (Second Military Medical University), Shanghai 200433, China;
3. Department of Neurosurgery, Changhai Hospital, Naval Medical University (Second Military Medical University), Shanghai 200433, China
*Corresponding author)
Abstract:
Objective To screen the differentially expressed long non-coding RNAs (lncRNAs) in glioblastoma, and to explore its role in the growth and invasion of glioblastoma. Methods Eight matched glioblastoma tumor tissues and peritumoral tissues were selected for high-throughput hybridization experiments, and the lncRNA with largest expression difference was screened. Human glioblastoma cell models with overexpression and interference expression of the lncRNA were established. Cell counting kit-8 (CCK-8) was used to detect the cell proliferation ability, flow cytometry was used to detect the apoptosis level, and Transwell assay was used to detect the cell invasion and migration abilities. Results The lncRNA MIR210HG, which was highly expressed in glioblastoma tissues, was screened out, and the glioblastoma cell models with overexpression and interference expression of lncRNA MIR210HG were successfully constructed. In vitro experiments showed that after overexpression of lncRNA MIR210HG, the apoptosis level of the glioblastoma cells was decreased, and the proliferation, invasion and migration abilities were increased, and after interference expression of lncRNA MIR210HG, the apoptosis level of the glioblastoma cells was increased, and the proliferation, invasion and migration abilities were decreased, with significant differences compared with control group (all P<0.05). Conclusion LncRNA MIR210HG can inhibit the apoptosis of glioblastoma cells, and promote cell proliferation, invasion and migration; and it may serve as a new target of glioblastoma therapy.
Key words:  glioblastoma|long non-coding RNAs|cell proliferation|apoptosis|cell invasion|cell migration